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OTUB2 is required for the growth of colorectal cancer. (A and B) Flow chart of the high-content screening (A), and the cell growth curves of the candidate OTUB2 after its silencing by using shOTUB2-derived lentivirus (lentivirus packaged in a mixed ratio of shOTUB2#1, shOTUB2#2 and shOTUB2#3) (B). (C) Silencing efficiency of indicated shRNAs by western blot. (D and E) HCT116 (D) and SW480 (E) cells were infected with indicated shRNAs-derived lentivirus for indicated times, followed by <t>trypan</t> <t>blue</t> <t>exclusion</t> <t>staining,</t> and cells were counted. (F) Tumor growth curves. (G and H) At the end of the animal study, tumors were excised, taken photos (G) and weighed (H). *P<0.05, **P<0.01, ***P<0.001; ##P<0.01, ###P<0.001.
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OTUB2 is required for the growth of colorectal cancer. (A and B) Flow chart of the high-content screening (A), and the cell growth curves of the candidate OTUB2 after its silencing by using shOTUB2-derived lentivirus (lentivirus packaged in a mixed ratio of shOTUB2#1, shOTUB2#2 and shOTUB2#3) (B). (C) Silencing efficiency of indicated shRNAs by western blot. (D and E) HCT116 (D) and SW480 (E) cells were infected with indicated shRNAs-derived lentivirus for indicated times, followed by <t>trypan</t> <t>blue</t> <t>exclusion</t> <t>staining,</t> and cells were counted. (F) Tumor growth curves. (G and H) At the end of the animal study, tumors were excised, taken photos (G) and weighed (H). *P<0.05, **P<0.01, ***P<0.001; ##P<0.01, ###P<0.001.
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OTUB2 is required for the growth of colorectal cancer. (A and B) Flow chart of the high-content screening (A), and the cell growth curves of the candidate OTUB2 after its silencing by using shOTUB2-derived lentivirus (lentivirus packaged in a mixed ratio of shOTUB2#1, shOTUB2#2 and shOTUB2#3) (B). (C) Silencing efficiency of indicated shRNAs by western blot. (D and E) HCT116 (D) and SW480 (E) cells were infected with indicated shRNAs-derived lentivirus for indicated times, followed by trypan blue exclusion staining, and cells were counted. (F) Tumor growth curves. (G and H) At the end of the animal study, tumors were excised, taken photos (G) and weighed (H). *P<0.05, **P<0.01, ***P<0.001; ##P<0.01, ###P<0.001.

Journal: American Journal of Cancer Research

Article Title: Inhibition of OTUB2 suppresses colorectal cancer cell growth by regulating β-Catenin signaling

doi:

Figure Lengend Snippet: OTUB2 is required for the growth of colorectal cancer. (A and B) Flow chart of the high-content screening (A), and the cell growth curves of the candidate OTUB2 after its silencing by using shOTUB2-derived lentivirus (lentivirus packaged in a mixed ratio of shOTUB2#1, shOTUB2#2 and shOTUB2#3) (B). (C) Silencing efficiency of indicated shRNAs by western blot. (D and E) HCT116 (D) and SW480 (E) cells were infected with indicated shRNAs-derived lentivirus for indicated times, followed by trypan blue exclusion staining, and cells were counted. (F) Tumor growth curves. (G and H) At the end of the animal study, tumors were excised, taken photos (G) and weighed (H). *P<0.05, **P<0.01, ***P<0.001; ##P<0.01, ###P<0.001.

Article Snippet: CRC cells were cultured for indicated times, and viable cells were evaluated by trypan blue exclusion staining (Beyotime, Beijing, China) or Cell Counting Kit-8 (CCK-8) assay (Selleck Chemicals, Houston, Texas, USA) following the manufacturers’ protocols.

Techniques: High Content Screening, Derivative Assay, Western Blot, Infection, Staining